SEPROT
  • CURSOS Y CONGRESOS
  • TRABAJO
  • BECAS
  • PUBLICACIONES
  • QUIÉNES SOMOS
  • SOCIOS

Article: Phosphoproteomic Analysis of Platelets in Severe Obesity Uncovers Platelet Reactivity and Signaling Pathways Alterations, A. García’s Lab (USC)

19 April, 2021Articulos Socios SEProt, Newsadmin

Phosphoproteomic Analysis of Platelets in Severe Obesity Uncovers Platelet Reactivity and Signaling Pathways Alterations
María N. Barrachina , Lidia Hermida-Nogueira , Luis A. Moran , Vanessa Casas , Sarah M. Hicks , Aurelio M. Sueiro , Ying Di , Robert K. Andrews , Steve P. Watson , Elizabeth E. Gardiner , Joaquin Abian , Montserrat Carrascal , María Pardo , Ángel García

Arteriosclerosis, Thrombosis and Vascular Biology volume 41:478-490 (2021);

Abstract:
Objective:
Obesity is associated with a proinflammatory and prothrombotic state that supports atherosclerosis progression. The goal of this study was to gain insights into the phosphorylation events related to platelet reactivity in obesity and identify platelet biomarkers and altered activation pathways in this clinical condition.
Approach and Results:
We performed a comparative phosphoproteomic analysis of resting platelets from obese patients and their age- and gender-matched lean controls. The phosphoproteomic data were validated by mechanistic, functional, and biochemical assays. We identified 220 differentially regulated phosphopeptides, from at least 175 proteins; interestingly, all were up-regulated in obesity. Most of the altered phosphoproteins are involved in SFKs (Src-family kinases)-related signaling pathways, cytoskeleton reorganization, and vesicle transport, some of them validated by targeted mass spectrometry. To confirm platelet dysfunction, flow cytometry assays were performed in whole blood indicating higher surface levels of GP (glycoprotein) VI and CLEC (C-type lectin-like receptor) 2 in platelets from obese patients correlating positively with body mass index. Receiver operator characteristics curves analysis suggested a much higher sensitivity for GPVI to discriminate between obese and lean individuals. Indeed, we also found that obese platelets displayed more adhesion to collagen-coated plates. In line with the above data, soluble GPVI levels—indicative of higher GPVI signaling activation—were almost double in plasma from obese patients.
Conclusions:
Our results provide novel information on platelet phosphorylation changes related to obesity, revealing the impact of this chronic pathology on platelet reactivity and pointing towards the main signaling pathways dysregulated.

Enlace: https://www.ahajournals.org/doi/10.1161/ATVBAHA.120.314485

Previous post Eight Spanish Proteomics Society – Juan Pablo Albar Award Next post Article: Integrative Multi-omics Analysis to Characterize Human Brain Ischemia from García-Berrocoso’s Lab (VHIR – CSIC – UAB)

ESTAMOS EN

Facebook
Twitter

LO ÚLTIMO

  • VII Meeting for young proteomics researchers
  • Article: Multiomics Profiling of Alzheimer’s Disease Serum for the Identification of Autoantibody Biomarkers. R Barderas’s Lab (ISCIII)
  • Article: Dysregulated protein phosphorylation: A determining condition in the continuum of brain aging and Alzheimer’s disease. E Santamaría’s Lab (UPNA-IdiSNA)

OFERTAS DE TRABAJO

  • Postdoc SUMO & Ubiquitin in the Cell Cycle – Leiden University Medical Center
  • Postdoc functional proteomics and cell signaling, Blagoy-Blagoev Lab, SDU – Denmark
  • Postdoc at MC Durán Lab, INIBICA-UCA, Cádiz

CURSOS Y CONGRESOS

  • VII Meeting for young proteomics researchers
  • Weminar: Infection Medicine, New-old approaches & perspectives

CONTACT

Sociedad Española de Proteómica C/. Jaime Roig 11 46010 Valencia
secretaria@seprot.es

ESTAMOS EN

Facebook
Twitter

SOCIAL NETWORKS

Facebook
Twitter

ARCHIVO

Desde 2005…

NEWS

  • VII Meeting for young proteomics researchers
  • Article: Multiomics Profiling of Alzheimer’s Disease Serum for the Identification of Autoantibody Biomarkers. R Barderas’s Lab (ISCIII)
  • Article: Dysregulated protein phosphorylation: A determining condition in the continuum of brain aging and Alzheimer’s disease. E Santamaría’s Lab (UPNA-IdiSNA)

LINKS

EuPa
Aviso Legal | Diseño Prisma
Esta web utiliza cookies para mejorar su experiencia Acepto Leer más
Política de cookies

Privacy Overview

This website uses cookies to improve your experience while you navigate through the website. Out of these, the cookies that are categorized as necessary are stored on your browser as they are essential for the working of basic functionalities of the website. We also use third-party cookies that help us analyze and understand how you use this website. These cookies will be stored in your browser only with your consent. You also have the option to opt-out of these cookies. But opting out of some of these cookies may affect your browsing experience.
Necessary
Always Enabled

Necessary cookies are absolutely essential for the website to function properly. This category only includes cookies that ensures basic functionalities and security features of the website. These cookies do not store any personal information.

Non-necessary

Any cookies that may not be particularly necessary for the website to function and is used specifically to collect user personal data via analytics, ads, other embedded contents are termed as non-necessary cookies. It is mandatory to procure user consent prior to running these cookies on your website.

SAVE & ACCEPT